Collagen Type II Cleavage Assay (C2C ELISA)
Cat. # 60-1001-001
Cat. # 60-1001-001
Intended use:
The C2C ELISA kit measures cartilage degradation. This assay with specific monoclonal antibody to type II collagen primary cleavage site recognizes the α-chain fragments containing an approximate 9 amino acid sequence from the carboxy terminus of the three-quarter length piece produced by collagenase cleavage (MMP-1, MMP-8, MMP-13) of type II collagen.
| Method: | ELISA competitive inhibition, 96 wells |
| Sample Type: | Optimized for serum (may also be used for plasma, synovial fluid, urine, cell culture, cartilage extracts) |
| Sample Volume: | 25 mcL per replicate |
| Calibration Range | 10 - 1000 ng/mL |
| Incubation Time: | 2.5 hours |
| Species: | Optimized for human (may also be used for various animal species) |
| Cross-Reaction | No cross-reaction with uncleaved triple-helical and heat-denatured human collagen types I and II and uncleaved alpha-chains of collage types I and II. No cross-reaction with correspondingly cleaved alpha-chains of collage type I. |
