Chondroitinase AC & Chondroitinase B

IBEX produces a family of high purity recombinant glycosaminoglycan (GAG) lyases; among them  Chondroitinase AC and Chondroitinase B (as well as Heparinase I, Heparinase II, Heparinase III).

The IBEX chondroitinases are widely used in research and as quality control reagents.

IBEX has developed a proprietary Flavobacterium heparinum  expression system which produces glycosylated enzymes exactly the same as GAGS  produced from the native strain, but with less cross-contaminating GAG enzymes

IBEX  chondroitinases do not contain external proteins such as bovine serum albumin (BSA), nor preservatives.

Catalyzed Reaction

The enzyme cleaves, via an elimination mechanism, sulfated and non-sulfated polysaccharide chains containing 1-4 linkages between hexosamines and glucuronic acid residues. The reaction yields oligosaccharide products (mainly disaccharides) containing unsaturated uronic acids which can be detected by UV spectroscopy at 232 nm. The enzyme is active on chondroitin sulfates A and C, chondroitin and hyaluronic acid, but is not active on dermatan sulfate (chondroitin sulfate B).

Catalyzed Reaction
The enzyme cleaves, via an elimination mechanism, polysaccharide chains containing 1-4 linkages between hexosamines and iduronic acid residues in dermatan sulfate (chondroitin sulfate B). The reaction yields oligosaccharide products (mainly disaccharides) containing unsaturated uronic acids which can be detected by UV spectroscopy at 232 nm.