Research Use ONLY
Aryl Acylamidase (native)
Aryl acylamidase (AAA), produced from P. fluorescens, catalyses the hydrolysis of acetaminophen (4-hydroxy acetanilide) producing 4-hydroxyaniline and acetate.
The enzyme activity is quantitated by spectrophotometrically measuring the rate of hydrolysis of 4-nitroacetanilide (PNAA) to yellow colored 4-nitroaniline (PNA) at 410 nm.